Mfold/UNAfold is useful for secondary structure prediction of RNAs.
Useful commands
mfold SEQ=`file_name' with optional parameters:
[ AUX=`auxfile_name' ] [ RUN_TYPE=text (default) or html ]
[ NA=RNA (default) or DNA ] [ LC=sequence type (default = linear) ]
[ T=temperature (default = 37˚) ] [ P=percent (default = 5) ]
[ W=window parameter (default - set by sequence length) ]
[ MAXBP=max base pair distance (default - no limit) ]
[ MAX=maximum number of foldings to be computed (default 100) ]
[ ANN=structure annotation (default = none, p-num or ss-count) ]
[ START=5' base # (default = 1)] [ STOP=3' base # (default = end) ]
[ REUSE=NO/YES (default=NO) reuse existing .sav file ]
References
http://unafold.rna.albany.edu/?q=mfold/download-mfold http://unafold.rna.albany.edu/doc/mfold-manual/node1.php